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Dual-channel chromatography a smart way to improve the analysis efficiency in liquid chromatography coupled to mass spectrometry

 L. Łukasz Rajskia, Florencia Jesúsb, Francisco José Díaz-Galianoa, Amadeo Rodríguez Fernández-Albaa

aEuropean Union Reference Laboratory for Pesticide Residues in Fruit & Vegetables. University of Almeria, Agrifood Campus of International Excellence ceiA3, Ctra. Sacramento s/n. La Cañada de San Urbano 04120-Almería, Spain
bGroup for the Analysis of Trace Compounds (GACT), Polo de Desarrollo Universitario Abordaje Holístico, CENUR Litoral Norte Sede Paysandú, Universidad de la República (UdelaR), Ruta 3 km 363, Paysandú, CP 60000, Uruguay

Journal of Chromatography A Volume 1633, 6 December 2020, 461614

DOI: https://doi.org/10.1016/j.chroma.2020.461614


Dual-channel chromatography was evaluated for pesticide residue analysis in fruits and vegetables and for unknown compounds detection. A dual-channel system was tested coupled to triple quadrupole and high-resolution mass spectrometry. The first part of the investigation was related to the improvement of the sample throughput with a 100 mm column. The dual-channel system provided the same analytical results as the single-channel system, however, with the throughput higher of about 70% (80 injections vs 137 injections in 24 h). Two types of calibration (in-channel and cross-channel) were checked. In the article, also solvent consumption is discussed. Six proficiency test samples were analysed to assess the quality of the results. Nor false positives neither false negatives were found. Calculated z-scores were typically <1. In the second part, a different approach was investigated. The 100 mm column was replaced by a 150 mm column keeping shorter run times than single channel system and 100 mm. The longer column improved the sensitivity and selectivity what was demonstrated in the target pesticide residue analysis. Additionally, the 150 mm column was compared with the 100 mm column in the analysis of unknown natural matrix compounds by high resolution mass spectrometry. The longer column allowed to detect up to 26% unknown compounds more than the shorter column.

© 2020 Elsevier B.V. All rights reserved.


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Published 30-10-2020, 09:22:46

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