Miniaturisation and optimisation of the Dutch mini-Luke extraction method for implementation in the routine multi-residue analysis of pesticides in fruits and vegetables

DOI:10.1016/j.foodchem.2015.07.065

Abstract

This paper presents the validation and further miniaturisation of the well-known Dutch mini-Luke method for high water and acid content matrices for 175 pesticides amenable to liquid– and gas chromatography– mass spectrometry. For optimisation of the method, recovery tests with different sample/extraction solvent ratios, varying amounts of dichloromethane and salts were performed with fifty representative pesticides. Solvent consumption could be reduced considerably, especially for the dichloromethane (by a factor of 3). Recovery studies performed with lettuce and orange matrix spiked at 0.005, 0.01 and 0.02 mg/kg yielded average recoveries in the range 70–120% with relative standard deviation values below 20% for almost all the pesticides tested. The linearity over three orders of magnitude was demonstrated (r2 > 0.99). The matrix effect could be considered as not significant. The limit of quantification was 0.005 mg/kg for 96% of the compounds. The optimised New Dutch mini-Luke (‘‘NL’’-) method was applied successfully in routine analysis and the EUPT FV-16 sample.

Copyright  2015 Published by Elsevier Ltd.

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Published 07-08-2015, 12:07:49